The Effect of Nitrogenous Fertilizers on Methane Oxidation in Soil

Emine Erman KARA, Hasan Göksel ÖZDİLEK

Ekoloji, 2010, Issue 74, Pages: 1-9


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One of the most prominent applications to increase agricultural yield is the application o f nitrogenous fertilizers to the soil. The intermediate and final products of the mineralization/humidification that include carbon dioxide (CO2), methane (CH4), and nitrous oxide (N2O) are released into the atmosphere while other final products are cycled into the soil upon completion of the related reactions that take place in soil. Released CO2 , CH4 , and N2O are the most notable factors responsible for global warming and are claimed to be remarkably serious when one accounts for all o f the agricultural lands and other similar fields all around the globe. In the present study, the impact o f NH4NO3 with 26% N, which consisted of 19% NH4-N (ammonium-N) and 7% NO3-N (nitrate-N), with and withouth nitrification inhibitor in the soil on CH4, CO2 and N2O gas emissions was monitored in a controlled laboratory environment as three parallel experiments. The amount o f fertilizer applied to the soil layer was 90 kg N per hectare. The two treated soils and one control soil were incubated at 60% maximal water holding capacity at 25°C during the test period of 18 days to investigate, (a) nitrogenous fertilizer (NH4NO3), (b) nitrogenous fertilizer plus nitrification inhibitor (NH4NO3 + N.I.), and finally (c) control for CH4, CO2, and N2O emissions. Gas compositions from the experimental soils were sampled at predetermined times in order to assess the CH4 release along with N2O and CO2 emissions. Analyses o f the headspace on the 1st, 2nd, 4th, 7th, 10th, 14th and 18th days were completed in order to evaluate gas concentrations. The overall results indicate that NH4NO3 fertilization and NH4NO3 with a nitrification inhibitor application causes a statistically significant decrease in CH4 emissions and an increase in CO2 emissions into the atmosphere. N 2O emissions were found to be statistically different with the NH4NO3 application and control treatments. Yet, no significant change was observed in, the N2O concentrations with respect to time, based on the NH4NO3 with a nitrification inhibitor application compared to the control group.


Carbon dioxide, methane oxidation, nitrogenous fertilizers, nitrous oxide, nitrification inhibitor, soil


  • Adams MB (2003) Ecological issues related to N deposition to natural ecosystems: research needs. Environment International 29, 189-199.
  • Bedard C, Knowles R (1989) Physiology, Biochemistry and Specific Inhibitors of CH4, NH4 and CO Oxidation by Methanotrophs and Nitrifiers. Microbiological Review 53, 68-84.
  • Bronson KF, Mosier AR (1994) Suppression of methane oxidation in aerobic soil by nitrogen fertilizers, nitrification inhibitors, and urease inhibitors. Biology and Fertility of Soils 37, 433-444.
  • Buendia L, Neue H, Wassmann R, Javellana LA (1997) Understanding the nature of methane emission from rice ecosystems as basis of mitigation strategies. Applied Energy 56, 433-444.
  • Bundy LG, Bremner JM (1973) Inhibition of nitrification in soils. Soil Science Society of America Proceedings 37, 396-398.
  • Cai ZC, Mosier AR (2000) Effects of NH4Cl addition on methane oxidation by paddy soils. Soil Biology and Biochemistry 32, 1537-1545.
  • Chang TC, Yang SS (2003) Methane emission from wetlands in Taiwan. Atmospheric Environment 37, 4551-4558.
  • Dan J, Kruger M, Frenzel P Conrad R (2001) Effect of late season urea fertilization on methane emissions from a rice field in Italy. Agriculture, Ecosystems and Environment 83, 191-199.
  • Denier Van der Gon HAC, Neue HU (1995) Influence of organic matter incorporation on the methane emission from a wetland rice field. Global Biogeochemistry 9, 11-22.
  • De Visscher A, Van Cleemput O (2003) Induction of enhanced CH4 oxidation in soils: NH4+ inhibition patterns. Soil Biology and Biochemistry 35, 907-913.
  • Fuhrer J (2003) Agroecosystem responses to combinations of elevated CO2, ozone and global climate change. Agriculture, Ecosystems and Environment 97, 1-20.
  • Hellebrand HJ, Kern J Scholz V (2003) Long term studies of greenhouse gas fluxes during cultivation of energy crops on sandy soils. Atmospheric Environment 37, 1635-1644.
  • Hütsch BW, Webster CP, Powlson DS (1993) Long term effects of nitrogen fertilization on methane oxidation in soil of the Broadbalk Wheat Experiment. Soil Biology and Biochemistry 25, 1307-1317.
  • Jäckel U, Schnell S, Conrad R (2001) Effect of moisture, texture and aggregate size of paddy soils on production and consumption of CH4. Soil Biology and Biochemistry 33, 965-971.
  • Khalil MAK, Rasmussen RA, Shearer MJ, Dalluge RW, Ren LX, Duan CL (1998) Measurements of methane emissions from rice fields in China. Journal of Geophysical Research 103, D-19, 25181-25210.
  • King GM (1992) Ecological Aspects of Methane Oxidation, a Key Determinant of Global Methane Dynamics. Advances in Microbial Ecology 12, 432-468.
  • Kravchenko I., Boeckx P, Galchenko V Van Cleemput O (2002) Short- and medium-term effects of NH4+ on CH4 and N 2O fluxes in arable soils with a different texture. Soil Biology and Biochemistry 34, 669-678.
  • Krupa S (2003) Atmosphere and environment in the new millennium. Environmental Pollution 126, 293-300.
  • Le Mer J, Roger P (2001) Production, oxidation, emission and consumption of methane by soils. European Journal of Soil Biology 37, 25-50.
  • Liou RM, Huang SN, Lin CW (2003) Methane emissions from fields with differences in nitrogen fertilizers and rice varieties in Taiwan paddy soils. Chemosphere 50, 237-246.
  • Lopez-Real J, Baptista M (1996) A preliminary comparative study of three manure composting systems and their influence on process parameters and methane emissions. Compost Science and Utilization 4, 71-82.
  • Maljanen M, Liikanen A, Silvola J, Martikainen PJ (2003) Methane fluxes on agricultural and forested boreal organic soils. Soil Use and Management 19, 73-79.
  • Neue HN, Wassmann R, Kludze H, Bujun W Lantin R (1997) Factors and processes controlling methane emissions from rice fields. Nutrient Cycling in Agroecosystems 49, 111-117.
  • Peigné J, Girardin P (2004) Environmental impacts of Farm-Scale composting practices. Water, Air and Soil Pollution 153, 45-68.
  • Priemé A, Ekelund F (2001) Five pesticides decreased oxidation of atmospheric methane in a forest soil. Soil Biology and Biochemistry 33, 831-835.
  • Prinn RG (1994) Global atmospheric-biospheric chemistry. In: Prinn RG (ed.) Global Atmospheric- Biospheric Chemistry, Plenum, New York, 1-39.
  • Sass RL, Fisher FM, Ding A, Huang Y (1999) Exchange of methane from rice fields: national, regional, and global budgets. Journal of Geophysical Research 104, 26943-26952.
  • Schnell S, King GM (1994) Mechanistic analysis of ammonium inhibition of atmospheric methane consumption in forest soil. Applied Environmental Microbiology 60, 3514-3521.
  • Steinkamp R, Butterbach Bahl K, Papen H (2001) Methane oxidation by soils of an N limited and N fertilized spruce forest in the Black Forest, Germany. Soil Biology and Biochemistry 33, 145-153.
  • Streese J, Stagmann R (2003) Microbial oxidation of methane from old landfills in biofilters. Waste Management 23, 573-580.
  • Willison TW, Webster CP Goulding KWT, Powlson DS (1995) Methane oxidation in temperate soils: Effects of land use and the chemical form of nitrogen fertilizer. Chemosphere 30, 539-546.
  • Wuebbles DJ, Hayhoe K (2002) Atmospheric methane and global change. Earth Science Review 57, 177-210.