Environmental Effect of Grouting Batches on Microbial-Induced Calcite Precipitation

Xing Gong, Jiuge Niu, Shihua Liang, Deluan Feng, Qingzi Luo

Ekoloji, 2019, Issue 107, Pages: 929-936, Article No: e107109

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Abstract

To gain an understanding of the environmental factors that affect the growth of the bacterium Sporosarcina pasteurii, the metabolism of the bacterium and the calcium carbonate precipitation induced by this bacterium to optimally implement the biological treatment process, microbial induced calcium carbonate precipitation (MICP), in situ. The grouting batches of nutrient solution and bacterial liquid significantly influence the curing effect of Microbial Induced Calcium carbonate Precipitation (MICP). This paper analyzed the physical and mechanical properties, and micro-structures of the sand cured by nutrient solution and bio-reaction fluids in different grouting batches, through the geotechnical test, and SEM, respectively, to explore the effect of grouting times on MICP. Grouting the bacterial of Sporosarcina pasteurii and nutrient solution intermittently, the permeability and water absorption of the bio-cemented sand decreased with the number of grouting batches, but the dry density, unconfined compression strength, and the amount of precipitated calcium carbonate increased. The apparent pore content and the average pore diameter of the SEM images also gradually decreased, indicating that the mechanical properties of the bio-cured sand have been improved. However, the solidification effect didn’t continuously increase after filling nutrient solution nine times in this study. The reason is that the concentration and activity of the residual bacteria, as well as the size and number of pores in the sand column, reduced with the increasing nutrient solution grouting batches, which slowed down the rate of precipitation reaction. Once the sand was solidified by cyclic grouting reaction fluids, the mechanical strength of the bio-cemented sand also increased with the cycle grouting batches. Since the average porosity of the sand column in the SEM image was less than 2% after cyclic filling the reaction fluids three times, the solidification effect changed in apparently with more grouting batches.

Keywords

MICP, grouting batches, sand, physical and mechanical properties, SEM, environmental factors

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