Effects of EDTA on Cr+3 Uptake, Accumulation, and Biomass in Nasturtium officinale (Watercress)

Didem Aydin, Omer Faruk Coskun

Ekoloji, 2013, Issue 87, Pages: 16-23

DOI: https://doi.org/10.5053/ekoloji.2013.873


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A pot study was used to examine the effects of ethylenediaminetetraacetic acid (EDTA) on the growth potential, uptake, and mobilization of Cr by Watercress (Nasturtium officinale) in contaminated water at different concentration levels of the chelating agent EDTA (0, 10-5, and 10-4 M) and four concentration levels of Cr (0, 1, 3, and 10 mgL-1). The EDTA resulted in more solubilization of Cr in water. The application of EDTA 15 d prior to harvest increased the amount of Cr accumulated in watercress with more Cr accumulated by the plants from the media. Nasturtium officinale accumulated high Cr concentration (317 mgkg-1) in the root at a concentration of 10 mgL-1 Cr3+ and 104- M EDTA after 15 d growth. The application of EDTA had inhibitory effects on the root and shoot dry biomass compared with that in the control. This plant can be used as potential species for chelate-assisted Cr phytoremediation.


biomass, chromium, EDTA, Nasturtium officinale, Phytoexraction


  • Akinci IE, Caliskan U (2010) Effect of Lead on Seed Germination and Tolerance Levels in Some Summer Vegetables. Ekoloji 19 (74): 164-172.
  • Arduini I, Masoni A, Ercoli L (2006) Effects of high chromium applications miscanthus during the period of maximum growth. Environmental and Experimental Botany 58:234–243.
  • Aslan M, Ünlü MY, Türkmen N, Yılmaz YZ (2003) Sorption of cadmium and effects on growth, protein content, and photosynthetic pigment composition of Nasturtium officinale R. Br. and Mentha aquatica L. Bulletin of Environmental Contamination and Toxicology 71: 323–329.
  • Bala R, Thukral AK (2008) Interactions between chromium and plant growth regulators on the growth of Spirodela polyrrhiza (L.) Schleiden. Terrestrial & Aquatic Environmental Toxicology 2: 14–18.
  • Chatterjee J, Chatterjee C (2000) Phytotoxicity of cobalt, chromium and copper in cauliflower. Environmental Pollution 109: 69-74.
  • Chen JC, Wang KS, Chen H, Lu CY, Huang LC, Li HC (2010) Phytoremediation of Cr(III) by Ipomonea aquatica (water spinach) from water in the presence of EDTA and chloride: Effects of Cr speciation. Bioresource Technology 101: 3033–3039.
  • Choo TP, Lee CK, Low KS, Hishamuddin O (2006) Accumulation of chromium(VI) from aqueous solution using water lilies (Nymphaea spontanea). Chemosphere 62: 961–967.
  • Demirayak A, Kutbay HG, Kilic D, Bilgin A, Huseyinova R (2011) Heavy Metal Accumulation in Some Natural and Exotic Plants in Samsun City. Ekoloji 20(79): 1-11.
  • Demirezen Yılmaz D (2007) Effects of salinity on growth and nickel accumulation capacity of Lemna gibba (Lemnaceae). Journal of Hazardous Materials 147: 74–77.
  • Dirilgen N (1998) Effects of pH and chelator EDTA on Cr toxicity and accumulation in Lemna minor. Chemosphere 37: 771–783.
  • Fu Y, Viraraghavan T (2001) Fungal decolorization of dye wastewaters: a review. Bioresource Technology 79: 251–262
  • Ghnaya T, Slama I, Messedi D, Grignon C, Abdelly C (2007) Cd-induced growth reduction in the halophyte Sesuvium portulacastrum is significantly improved by NaCl. Journal of Plant Research 120: 309-316.
  • Grcman H, Vodnik D, Velikonja-Bolta, Le tan, D (2003) Ethylenediaminedissuccinate as a new chelate for environmentally safe enhanced lead phytoextraction. Journal of Environmental Quality 32: 500-506.
  • Hanna F, Hamid ZA, Aal AA (2004) Controlling factors affecting the stability and rate of electroless copper plating. Materials Letters 58: 104-109.
  • Hoagland DR, Snyder WC (1993) Nutrition of strawberry plant under controlled conditions: (1) effects of deficiencies of boron and certain other elements: (b) susceptibility to injury from sodium salts. Proceedings of the American Society for Horticultural Sciene 30: 288–294.
  • Huang H, Li T, Tian S, Gupta DK, Zhang X, Yang X (2008) Role of EDTA in alleviating lead toxicity in accumulator species of Sedum alfredii H. Bioresource Technology 99: 6088–6096.
  • Hunt R (1990) Basic growth analysis. Plant Growth Analysis for beginners. Unwin in Hyman, London.
  • Huseyinova R, Kutbay HG, Bilgin A, Kılıc D, Horuz A, Kirmanoglu C (2009) Sulphur and Some Heavy Metal Contents in Foliage of Corylus avellana and Some Roadside Native Plants in Ordu Province, Turkey. Ekoloji 18(70): 10-16.
  • January MC, Cutright TJ, Keulen HV, Wei R (2008) Hydroponic phytoremediation of Cd, Cr, Ni, As, and Fe: can Helianthus annus hyperaccumulate multiple heavy metals. Chemosphere 70: 531–537.
  • Jean L, Bordas F, Bollinger JC (2007) Chromium and nickel mobilization from a contaminated soil using chelants. Environmental Pollution 147: 729–736.
  • Kumar NPBA, Dushenkov V, Motto H, Raskin I (1995) Phytoextraction: The use of plants to remove heavy metals from soils. Environmental Science & Technology 29: 1232-1238.
  • Knudson LL, Tibbitts WT, Edwards EG (1977) Measurement of ozone injury determination of leaf chlorophyll concentration. Plant Physiology 60: 606-608.
  • Li T, Yang X, Lu L, Islam E, He Z, (2009) Effects of zinc and cadmium interactions on root morphology and metal translocation in a hyperaccumulating species under hydroponic conditions. Journal of Hazardous Materials 169: 734–741
  • Luo C, Shen Z, Lou L, Li X (2006) EDDS and EDTA-enhanced phytoextraction of metals from artificially contaminated soils and residual effects of chelant compounds. Environmental Pollution 144: 862–871.
  • Maine MA, Suñé NL, Lagger SC (2004) Chromium bioaccumulation: comparison of the capacity of two floating aquatic macrophytes. Water Research 38: 1494–1501.
  • Meighan MM, Fenus T, Karey E, MacNeil J (2011) The impact of EDTA on the rate of accumulation and root/shoot partitioning of cadmium in mature dwarf sunflowers. Chemosphere 83(11):1539-1545.
  • Paiva LB, Oliveir JG, Azevedo RA, Ribeiro DR, Silva MG, Vitoria AP (2009) Ecophysiological responses of water hyacinth exposed to Cr3+ and Cr6+. Environmental and Experimental Botany 65: 403–409.
  • Panda SK (2007) Chromium-mediated oxidative stress and ultrastructural changes in root cells of developing rice seedlings. Journal of Plant Physiology 164: 1419–1428.
  • Rahmani GNH, Sternberg SPK (1999) Bioremoval of lead from water using Lemna minor. Bioresource Technology 70: 225–230.
  • Ruley AT, Sharma NC, Sahi SV, Singh SR, Sajwan KS (2006) Effects of lead and chelators on growth, photosynthetic activity and Pb uptake in grown in soil. Environmental Pollution 144: 11-18.
  • Saygideger S, Dogan M (2005) Influence of pH on lead uptake, chlorophyll and nitrogen content of Nasturtium officinale R. Br. and Mentha aquatica L. Journal of Environmental Biology 26:753–759.
  • Shanker AK, Cervantes C, Loza-Tavera H, Avudainayagam S (2005) Chromium toxicity in plants. Environment International 31: 739–753.
  • Suñe N, Sánchez G, Caffaratti S, Maine MA (2007) Cadmium and chromium removal kinetics from solution by two aquatic macrophytes. Environmental Pollution 145: 467–473.
  • Turgut C, Katie Pepe M, Cutright TJ (2004) The effect of EDTA and citric acid on phytoremediation of Cd, Cr, and Ni from soil using Helianthus annuus. Environmental Pollution 131:147-154.
  • Xiong ZT, Li YH, Xu B (2002) Nutrition influence on copper accumulation by Brassica pekinensis Rupr. Ecotoxicology and Environmental Safety 53: 200–205.
  • Wang KS, Huang LC, Lee HS, Chen PY, Chang SH (2008) Phytoextraction of cadmium by Ipomoea aquatica (water spinach) in hydroponic solution: effects of cadmium speciation. Chemosphere 72: 666–672.
  • Wu LH, Luo YM, Xing XR, Christie P (2004) EDTA-enhanced phytoremediation of heavy metal contaminated soil with Indian mustard and associated potential leaching risk. Agriculture, Ecosystems & Environment 102: 307–318.
  • Yu XZ, Gu JD, Huang SZ (2007) Hexavalent chromium induced stress and metabolic responses in hybrid willows. Ecotoxicology 16: 299–309.
  • Zayed AM, Terry N (2003) Chromium in the environment: factors affecting biological remediation. Plant and Soil 249: 139–156.
  • Zurayk R, Sukkariyah B, Baalbaki R, Ghanem DA (2001) Chromium phytoaccumulation from solution by selected hydrophytes. International Journal of Phytoremediation 3: 335-350.