Consortium of Hydrocarbon-Oxidizing Microorganisms as a Basis for a Biological Product for Treating Petroleum Industry Waste in Southern Kazakhstan

Akmaral U. Issayeva, Aigul A. Uspabayeva, Aigul M. Sattarova, Zhadra A. Shingisbayeva, Raziya A. Isaev

Ekoloji, 2017, Issue 100, Pages: 1-10, Article No: e100001

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Abstract

In order to design a new biological product for treating oil-polluted soil in the arid climate of Kazakhstan, conducted an extensive search for promising oil-oxidizing bacteria that were well resistant to various adverse environmental factors. Strains of petroleum product decomposers Rhodococcus erythropolis DP 304-B7 and Micrococcus varians N 313-S14 could serve as a basis for a biological product. The fact that the studied strains lacked virulence, toxicity, toxigenicity, and ability to invade internal organs of laboratory animals was indicative of their nonpathogenic nature and the possibility of using them in natural conditions.

Keywords

bioremediation, microflora, soil, petroleum

References

  • Achuba FI, Okoh PN (2014) Effect of petroleum products on soil catalase and dehydrogenase activities. Open Journal of Soil Science, 4(12): 399.
  • Adams FV, Niyomugabo A, Sylvester OP (2017) Bioremediation of Gruide oil contaminated soil using agricultural wastes. Procedia Manufacturing, 7: 459–464.
  • Al–Saleh E, Drobiova H, Obuekwe C (2009) Predominant culturable crude oil–degrading bacteria in the coast of Kuwait. Int. Biodeterior. Biodegradation, 63(4): 400–406.
  • Alvarez HM, Silva RA, Herrero M, Hernández MA, Villalba MS (2013) Metabolism of triacylglycerols in Rhodococcus species: insights from physiology and molecular genetics. Journal of Molecular Biochemistry, 2(1).
  • Arif NM, Ahmad SA, Syed MA, Shukor MY (2012) Isolation and characterization of a phenol-degrading Rhodococcus sp. strain AQ5NOL 2 KCTC 11961BP. Journal of Basic Microbiology, 53(1): 9-19.
  • Bouyocos GH (1951) A recalibration of the hydrometer for making mechanical analysis of soils. Agron. J., 43: 434-438.
  • Carter MR, Gregorich EG (2007) Soil sampling and methods of analysis. Boca Raton, Florida: CRC Press, 1224.
  • Chowdhury S, Bala NN, Dhauria P (2012) Bioremediation: a natural way for cleaner environment. Inter J Pharm Chem & Biol Sci., 2: 600–611.
  • Cowan ST (1974) Cowan and Steel’s manual for the identification of medical bacteria. Second edition. Cambridge University press.
  • García–Martínez AM, Tejada M, Díaz A, Bautista JD, Rodríguez B, Parrado J (2010) Enzymatic production of an organic soil biostimulant from wheat–condensed distiller solubles: effects on soil biochemistry and biodiversity. Process Biochem, 45: 1127–1133.
  • Ghosh A, Khurana M, Chauhan A, Takeo M, Chakraborti AK, Jain RK (2010) Degradation of 4-Nitrophenol, 2-Chloro-4-Nitrophenol, and 2, 4-Dinitrophenol by Rhodococcus imtechensis Strain RKJ300. Environ. Sci. Technol., 44(3): 1069–1077.
  • Hamamura N, Olson SH, Ward DM, Inskeep WP (2008) Assessing soil microbial populations responding to crude-oil amendment at different temperatures using phylogenetic, functional gene (alkB) and physiological analyses. Environ. Sci.Technol., 42(20): 7580-7586.
  • Hamamura N, Olson SH, Ward DM, Inskeep WP (2006) Microbial population dynamics associated with crude-oil biodegradation in diverse soils. Applied and Environmental Microbiology, 72(9): 6316-6324.
  • Ichor T, Gberikon GM, Nevkaa D (2016) Biodegradation of Phenanthrene by a Consortium of Aerobic Heterotrophic Bacteria and Cyanobacteria in Petroleum Hydrocarbon Polluted Brackish Water of Bodo Creek. Microbiology Journal, 6: 1-8.
  • Janbandhu A, Fulekar MH (2011) Biodegradation of Phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment. J. Hazard. Mater., 187: 333-340.
  • Juhasz AL, Naidu R (2000) Bioremediation of high molecular weight polycyclic aromatic hydrocarbons: a review of the microbial degradation of benzo[a]pyrene. Int. Biodeterior. Biodegradation, 45(1–2): 57–88.
  • Keil D, Meyer A, Berner D, Poll C, Schützenmeister A, Piepho H–P, Vlasenko A, Philippot L, Schloter M, Kandeler E (2011) Influence of land–use intensity on the spatial distribution of N–cycling microorganisms in grassland soils. FEMS Microbiol. Ecol., 77(1): 95–106.
  • Kiran H, Udiwal VM (2010) Restoration of Oil Contaminated Soil by Bioremediation for Ground Water Management and Environment Protection. IJCEPR, 1(1): 17-26.
  • Kirimura K, Furuya T, Sato R, Ishii Y, Kino K, Usami S (2002) Biodesulfurization of Naphthothiophene and Benzothiophene through Selective Cleavage of Carbon–Sulfur Bonds by Rhodococcus sp. Strain WU–K2R. Appl. Environ. Microbiol., 68(8): 3867–3872.
  • Kuyukina MS, Ivshina IB (2010) Rhodococcus biosurfactants: biosynthesis, properties, and potential applications. In Biology of Rhodococcus. Springer, Berlin, Heidelberg: 291-313.
  • Martínková L, Uhnáková B, Pátek M, Nešvera J, Ken V (2009) Biodegradation Potential of the Genus Rhodococcus. Environment International, 35(1): 162–177.
  • Montgomery DC (2009) Statistical Quality Control: A Modern Introduction (Sixth Edition). John Wiley & Sons.
  • Norazah MN, Ahmad SA, Ibrahim AL (2017) Effect of different phenol concentration on phenol degradation by Rhodococcus spp. Indian Journal of Fundamental and Applied Life Sciences, 7(1): 39-44.
  • Olsen SR (1954) Estimation of available phosphorus in soils by extraction with sodium bicarbonate. United States Department of Agriculture; Washington, 939.
  • Païssé S, Goñi-Urriza M, Coulon F, Duran R (2010) How a bacterial community originating from a contaminated coastal sediment responds to an oil input. Microbial ecology, 60(2): 394–405.
  • Pathak A, Green SJ, Ogram A, Chauhan A (2013) Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential. Genome Announcements, 1(1): e00144-12.
  • Ramadan MA, Hashem AM, Amin MA, Rfky NH (2012) Immobilization and Surfactant Enhanced Anthracene Biodegradation in Soil. Journal of American Science, 8(3): 596-602.
  • Robrock KR, Mohn WW, Eltis LD, Alvarez-Cohen L (2011) Biphenyl and Ethylbenzene Dioxygenases of Rhodococcus jostii RHA1 Transform PBDEs. Biotechnol. Bioeng., 108(2): 313–321.
  • Ruggeri C, Franzetti A, Bestetti G, Caredda P, La Colla P, Pintus M, ... Tamburini E (2009) Isolation and characterisation of surface active compound-producing bacteria from hydrocarbon-contaminated environments. International biodeterioration & biodegradation, 63(7): 936-942.
  • Sankaram A (1996) A Laboratory manual for agricultural chemistry. Asia publishing house, New Dehli, 340.
  • Santhini K, Myla J, Sajani S, Usharani G (2009) Screening of Micrococcus Sp from Oil Contaminated Soil with Reference to Bioremediation. Botany Research International, 2(4): 248-252.
  • Silva RA, Grossi V, Olivera NL, Alvarez HM (2010) Characterization of indigenous Rhodococcus sp. 602, a strain able to accumulate triacylglycerides from naphthyl compounds under nitrogen-starved conditions. Research in Microbiology, 161(3): 198.
  • Solyanikova I, Golovleva L (2011) Biochemical features of the degradation of pollutants by Rhodococcus as a basis for contaminated wastewater and soil cleanup. Microbiology, 80(5): 591.
  • Wang J, Xu H, An M, Yan G (2008) Kinetics and characteristics of phenanthrene degradation by a microbial consortium. Petroleum Sci., 5: 73-78.