Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp.

Sevim Feyza ERDOGMUS, Safiye Elif KORCAN, Muhsin KONUK, Kıymet GUVEN, Mehmet Burcin MUTLU

Ekoloji, 2015, Issue 94, Pages: 10-16

DOI: https://doi.org/10.5053/ekoloji.2015.942

OPEN ACCESS

Download Full Text (PDF)

Abstract

The aim of this study is to reveal the ability of utilizing different aromatic hydrocarbons (p-hydroxybenzoic acid, naphthalene, phenanthrene, and pyrene) by a halotolerant bacterial strain, Chromohalobacter sp., under saline conditions. The aromatic hydrocarbon degradation pathways were identified. PCR amplification was carried to define the gene zones which codify the dioxygenases of the isolates. The possible gene zones of catechol 1, 2 dioxygenase and protocatechuate 3, and 4 dioxygenase were determined. According to the PCR amplification and enzyme test results Chromohalobacter sp. utilizes aromatic hydrocarbons by the ortho cleavage of the ß-ketoadipate pathway. In this study, it was concluded that this isolate can be used in bioremediation studies of saline environments contaminated with aromatic hydrocarbons.

Keywords

Chromohalobacter sp., catechol 1, 2 dioxygenase, protocatechuate 3,4 dioxygenase

References

  • Arulazhagan P, Vasudevan N (2011) Role of nutrients in the utilzation polycyclic aromatic hydrocarbons by halotolerant bacterial strain. Journal of Environmental Science (China) 23(2): 282-287.
  • Arulazhagan P, Vasudevan N (2009) Role of a moderately halophilic bacterial consortium in the biodegradation of polyaromatic hydrocarbons. Marine Pollution Bulletin 58(2): 256-262.
  • Boonchan S, Britz ML, Stanley GA (2000) Degradation and mineralization of high molecular weight polycyclic aromatic hydrocarbons by defined fungal-bacterial cocultures. Applied of Environmental Microbiology 66: 1007-1019.
  • Borgne SL, Paniagua D, Vazquez-Duhalt R (2008) Biodegradation of organic pollutants by halophilic bacteria and archaea. Journal of Molecular Microbiology and Biotechnology 15: 74-92.
  • Bradford MM (1976) A rapid and sensitive metod for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Analytical Biochemistry 72: 248-258.
  • Dastgheib SM, Amoozegar MA, Khajeh K, Shavandi M, Ventosa A (2012) Biodegradation of polycyclic aromatic hydrocarbons by a halophilic microbial consortium. Applied Microbiology and Biotechnology 95(3): 789-798.
  • Erdoğmuş SF, Mutlu B, Korcan SE, Güven K, Konuk M (2013) Aromatic hydrocarbon degradation by halophilic archaea isolated from Çamaltı Saltern, Turkey. Water Air and Soil Pollution 224(1449): 1-9. Doi: 10.1007/s11270-013-14.
  • Fetzner S, Muller R, Lingens F (1989) Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS. Biological Chemistry Hoppe-Seyler 370: 1173-1182.
  • Fu W, Oriel P (1999) Degradation of 3-phenylpropionic acid by Haloferax sp. D1227. Extremophiles 3: 45-53.
  • Garcia MT, Ventosa A, Mellado E (2005) Catabolic versatility of aromatic compound degrading halophilic bacteria. FEMS Microbiology Ecology 1: 97-109.
  • Hegeman GD (1966) Synthesis of enzymes of the mandelate pathways by Pseudomonas putida, Synthesis of enzyme by the wild type. Journal of Bacteriology 91: 1140-1154.
  • Kim D, Kim SI, Choi KY, Lee JS, Kim E (2008) Molecular cloning and functional characterization of the genes encoding benzoate and p-hydroxybenzoate degradation by the halophilic Chromohalobacter sp. strain HS-2. FEMS Microbiology Letters 280: 235-241.
  • Lazaroaie MM (2008) Adaptative response of Chromohalobacter salexigens DSM 3043 to saturated and aromatic hydrocarbons. Electronic Journal of Biology 4(3): 120-128.
  • Moreno M, Sanchez-Porro C, Piubeli F, Frias L, Garcia MT (2011) Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans. Plos One 6: 1- 8.
  • Ngai KL, Neidle EL, Ornston LH (1990) Catechol and chlorocatechol 1,2-dioxygenases. Methods of Enzymology 188: 122-126.
  • Nicholson CA, Fathepure BZ (2004) Biodegradation of benzene by halophilic and halotolerant bacteria under aerobic conditions. Applied of Environmental Microbiology 70: 1222-1225.
  • Oren A (2002) Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. Journal of Industrial Microbiology and Biotechnology 28: 56-63.
  • Ottow JCG, Zolg W (1974) Improved procedure and colorimetrics test for the detection of ortho and meta cleavage of protocatechuate by Pseudomonas isolates. Canadian Journal of Microbiology 20: 1059-1061.
  • Parilti NB (2010) Treatment of a petrochemical industry wastewater by a solar oxidation process using the box-wilson experimental design method. Ekoloji 19(77): 9-15.
  • Rodrigues DF, Sakata SK, Comasseto JV, Bicego MC, Pellizari VH (2008) Diversity of hydrocarbon- degrading Klebsiella strains isolated from hydrocarbon contaminated estuaries. Journal of Applied Microbiology 106: 1304-1314. Doi:10.1111/j.1365-2672.2008.04097.x
  • Rodriguez VF, Ventosa A, Juez G, Imhoff JF (1985) Variation of environmental features and microbial populations with salt concentrations in a multi pond saltern. Microbial Ecology 11: 107-115.
  • Salmanov M, Aliyeva S, Veliyev M, Bekrashi N (2008) The study of degradation ability of oil products and oil hydrocarbons by microscopic fungi isolated from polluted coastal areas of absheron peninsula of caspian sea. Ekoloji 17(68): 59-64.
  • Sarma SD, Arora P (2001) Halophiles in: Encyclopedia of Life Sciences. Nature Publishing Group 1-9. DasSarma S, Arora P (2001) Halophiles. In: Encyclopedia of Life Sciences. Nature Publishing Group London, 1-9.
  • Stanier RY, Ingraham JL (1954) Protocatechuic acid oxidase. Journal of Biological Chemistry 210: 799-808. Vargas C, Nieto JJ (2004) Genetic tools for the manipulation of moderately halophilic bacteria of the family Halomonadaceae. Methods in Molecular Biology 267: 183-208.
  • Ventosa A, Nieto JJ, Oren A (1998) Biology of moderately halophilic aerobic bacteria. Microbiology and Molecular Biology Reviews 62: 504-544.
  • Vogel TM (1996) Bioaugmentation as a soil bioremediation approach. Current Opinion in Biotechnology 7: 311-316.
  • Zhao B, Wang H, Mao X, Li R (2009) A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons. Letters in Applied Microbiolgy 49: 408-410.